FIG. 6.

(A) 293T cells were transfected with the indicated amounts of a JAK1 expression vector and with empty vector (−) or a vector for nsp1 (+), and the levels of JAK1 were determined by immunoblotting. (B) 293T cells transfected with empty vector (−) or vector for JAK1 or TYK2, with or without nsp1, were treated with 2,000 U/ml of IFN-α for 0, 10, 30, and 60 min, and extracts were analyzed by immunoblotting. To compensate for the effect of nsp1 on JAK1 levels, 10 μl of extracts transfected with 7 μg of JAK1 and 30 μl of extracts cotransfected with 15 μg of JAK1 and 15 μg of nsp1 were loaded in the gel.