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Results of annexin V and PI staininga
| Vector for cell transfection | % Stained cells
|
|||
|---|---|---|---|---|
| Annexin V− PI+ (dead) | Annexin V+ PI+ (late apoptosis/ necrosis) | Annexin V− PI− (live) | Annexin V+ PI− (early apoptosis) | |
| Empty vector | 1.28 | 1.61 | 91.12 | 5.99 |
| nsp1 vector | 0.03 | 0.12 | 97.44 | 2.41 |
293T cells were transfected with empty vector or a vector for nsp1, together with pcDβA-eGFP, for 48 h, and unfixed cells were stained with annexin V-allophycocyanin and PI exactly as described previously (43).
