FIG. 4.

Reactivation by superinfection. HFFF2 cultures containing quiescent virus were established after infection with in1374 at an MOI of 3 (top), 0.3 (middle), or 0.03 (bottom). At 8 days p.i., the cultures were superinfected (si) with dl1403YR (lanes 1 to 6) or dl1403Y (lanes 7 to 12). After a 1-h adsorption period, the cells were acid washed and incubated at 38.5°C for 22 h. Cellular DNA was prepared, cleaved with BamHI, and probed with radiolabeled VP16 coding region from pMC17, as described in the legend to Fig. 3. The MOI of the superinfecting virus was 0.1 (lanes 1 and 7), 0.3 (lanes 2 and 8), 1 (lanes 3 and 9), 3 (lanes 4 and 10), 10 (lanes 5 and 11), or 30 (lanes 6 and 12).