FIG. 5.

Coinfection of in1374 with dl1403Y or dl1403YR. HFFF2 monolayers were incubated at 38.5°C for 8 days, infected with in1374 at an MOI of 3 (top) or 0.3 (bottom), and coinfected with dl1403YR (lanes 1 to 6) or dl1403Y (lanes 7 to 12). After a 1-h adsorption period, the cells were acid washed and incubated at 38.5°C for 22 h. Cellular DNA was prepared, cleaved with BamHI, and probed with radiolabeled VP16 coding region from pMC17, as described in the legend to Fig. 3. The MOI of the coinfecting virus was 0.1 (lanes 1 and 7), 0.3 (lanes 2 and 8), 1 (lanes 3 and 9), 3 (lanes 4 and 10), 10 (lanes 5 and 11), or 30 (lanes 6 and 12).