FIG. 2.

Representative primer extension analysis of variants. Replicative intermediates of WT reference and variants were isolated from cytoplasmic capsids from Huh7 cells and analyzed by primer extension to measure the levels of pgRNA and minus-strand DNA. The oligonucleotides 1661⁺ and 1948⁻ were used. The positions of the 5′ ends of pgRNA and minus-strand DNA are indicated by arrows on the left. The single asterisk indicates the altered positions at which minus-strand DNA migrates reflecting the size of the deletion. The double asterisks indicate the altered position at which pgRNA migrates, reflecting the size of the deletion. Oligonucleotide 1556⁺ was used instead of 1661⁺ to measure levels of minus-strand DNA for Δ1604-1674 and Δ1675-1744 (lanes 11 and 12).