TABLE 1.
Phenotypic and functional analysis of CD8 T cells following vaccination with AAV2/8 Gag
| Cell type/cell population | % Expression of phenotype or function in cell populationa
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Peak
|
Long term
|
|||||||
| PBMC | Spleen | Liver | Lung | PBMC | Spleen | Liver | Lung | |
| Tetramer+/CD8 | 40.8 ± 7.2 | 34.6 ± 3.7 | 79.7 ± 2.6 | 71 | 11.2 ± 5.52 | 7.51 ± 3.19 | 50.1 ± 8.58 | 66 |
| TE+/tetramer | 99.55 | 99.19 | 97.91 | 99.63 | 94.68 | 97.19 | 94.69 | 94.46 |
| TEM+/tetramer | 0.43 | 0.76 | 1.62 | 0.29 | 4.88 | 2.67 | 2.43 | 5.21 |
| TCM+/tetramer | 0.03 | 0.05 | 0.48 | 0.08 | 0.44 | 0.14 | 2.87 | 0.34 |
| GrB+ Ter+/CD8 | − | 8.24 | 75.06 | − | − | 1.07 | 41.40 | − |
| IFN-γ+/CD8 | − | 7.69 ± 0.54 | 14.7 ± 7.19 | 13.01 | − | 1.82 ± 0.84 | 5.30 ± 1.26 | 2.61 |
| TNF-α+/CD8 | − | 0.14 ± 0.06 | 0.76 ± 0.15 | 0.87 | − | 0.04 ± 0.05 | 0.69 ± 0.25 | 2.48 |
| IFN-γ+ TNF-α+/CD8 | − | 4.12 ± 1.51 | 9.82 ± 0.71 | 14.8 | − | 2.97 ± 2.70 | 6.68 ± 1.86 | 24.37 |
| IFN-γ+ TNF-α+ IL-2+/CD8 | − | 0.05 ± 0.04 | 0.12 ± 0.02 | 0.054 | − | 0.05 ± 0.05 | 0.01 ± 0.01 | 0.27 |
a
−, not measured; Ter, tetramer.