FIG. 1.

Both CK2α and -β subunits copurify with the 184 EB2 N-terminal amino acids. HEK293T cells were transfected with pNTAP.EB2Nter, expressing the EB2Nter protein fused at the N terminus to a calmodulin-binding peptide and to a streptavidin-binding peptide. TAP-tagged EB2 and associated proteins expressed in HEK293T cells were sequentially purified on streptavidin and calmodulin columns. The purified proteins were separated by 2D gel, silver stained, and identified by MALDI-TOF mass spectrometry. The unlabeled spots were not identified by mass spectrometry. The positions of the molecular mass markers are indicated on the left of the gel. pI, isoelectric point.