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EB2 and CK2β colocalize in the nuclei of living cells. HeLa cells expressing F.EB2 were immunostained using a rabbit anti-CK2β antibody and an Alexa Fluor 488-conjugated secondary antibody (B) and the M2 anti-Flag MAb and a Fluorolink Cy3-conjugated secondary antibody (C). (D) Colocalization of CK2β and EB2. The cell fluorescence was examined using confocal microscopy. Panel A shows the same cells in phase-contrast microscopy.
