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Purified CK2 holoenzyme phosphorylation sites are restricted to the N-terminal 80 amino acids of EB2. Glutathione-Sepharose 4B beads loaded with GST-Nter, -A, -B, and -C were incubated with purified CK2 holoenzyme in an in vitro kinase assay. The GST fusion proteins were eluted, resolved by SDS-PAGE, and analyzed by autoradiography.
