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. 2007 Aug 22;81(21):11946–11956. doi: 10.1128/JVI.00620-07

FIG. 3.

FIG. 3.

Effect of CsA treatment of target cells on reverse transcription (RT) of wild-type and mutant HIV-1 particles. VSV-G-pseudotyped HIV-1-GFP viruses were produced by transfection of 293T cells, normalized for p24, and used to inoculate 293T (A, B, and C) and HeLa-P4 (D, E, and F) cells in the presence or absence of 5 μM CsA. DNA was isolated from target cells at 6 and 12 h postinfection and assayed for early (A and D) and late (B and E) products of reverse transcription by quantitative PCR. As a control, the wild-type virus (WT) was inoculated in the presence of the reverse transcriptase inhibitor Efavirenz (WT/E). (C and F) Parallel cultures were analyzed 2 days postinoculation for GFP expression by flow cytometry. Both assays were performed with a high dose of virus to maximize the likelihood of detection of viral DNA in 293T and HeLa-P4 cells. Results are representative of two independent experiments.