FIG. 5.

Effect of CsA on HIV-1 infection of 293T-HeLa-P4 heterokaryons. Heterokaryons were produced by coculture of DDAO-stained 293T cells expressing the M-PMV Env glycoprotein with CMTMR-stained HeLa-P4 cells. Cultures were inoculated with the indicated GFP reporter viruses in the presence or absence of CsA. One-half million cells from each culture were analyzed by three-color flow cytometry for GFP, DDAO, and CMTMR. (A) Analysis of control uninfected cultures lacking M-PMV Env expression. (B) Analysis of heterokaryon-containing cultures. (C to F) Analysis of GFP expression in 293T cells (C), HeLa-P4 cells (D), and heterokaryon cells (E) in the presence or absence of CsA. For panel F, cultures of heterokaryons in which the DDAO and CMTMR staining was reversed were analyzed. Results are representative of two independent experiments. WT, wild type.