FIG. 3.

mLANA-deficient virus exhibits increased kinetics of cell death and PARP cleavage. (A and B) NIH 3T3 fibroblasts were mock infected or were infected with 73.Stop or 73.MR virus at an MOI of 2 PFU/cell and were harvested 24 h postinfection. (A) Representative images of cells that were fixed and stained with crystal violet for imaging by light microscopy. (B) Cell viability of triplicate samples determined by trypan blue exclusion. A minimum of 200 cells was counted per sample. Results are the means of triplicate samples. Error bars represent standard deviations. (C) NIH 3T3 fibroblasts were mock infected or were infected with 73.Stop or 73.MR virus at an MOI of 2 PFU/cell and were harvested at the indicated times postinfection. Equivalent amounts of total protein were resolved by SDS-PAGE, and the integrity of PARP was determined by immunoblotting. Arrows denote intact (upper) or cleaved (lower) PARP.