FIG. 4.
73.Stop virus exhibits increased replication, viral antigen expression, and p53 induction. (A) MEFs were infected with 73.Stop or 73.MR virus at an MOI of 2 PFU/cell, and cells were harvested at the indicated times postinfection. Viral yields were determined by plaque assay. Results are the means from triplicate samples. Error bars represent standard deviations. P values were determined using a two-tailed paired Student's t test. (B) NIH 3T3 fibroblasts were infected with 73.Stop or 73.MR virus at an MOI of 2 PFU/cell. Equivalent numbers of cells were harvested directly by addition of Laemmli sample buffer at the indicated times postinfection. Total cell lysates were resolved by SDS-PAGE and were analyzed by immunoblotting with antibodies to the indicated proteins. Untreated and UV-exposed cells (100 J/m2) serve as negative and positive controls, respectively, for p53 stabilization and activation. The blot shown is representative of three independent experiments.