FIG. 7.
p53 regulates MHV68 replication. p53comp MEFs (A) or p53−/− MEFs (B) were infected with 73.Stop or 73.MR virus at an MOI of 0.001 PFU/cell. Cells were harvested at the indicated times postinfection, and viral titers were determined by plaque assay. Results are the means from triplicate samples. Error bars represent standard deviations. (C) WT or p53−/− MEFs were infected with WT, 73.Stop, or 73.MR MHV68 at an MOI of 2 PFU/cell. Equivalent numbers of cells were harvested directly by addition of Laemmli sample buffer at the indicated times postinfection. Total cell lysates were resolved by SDS-PAGE and were analyzed by immunoblotting with antibodies to the indicated proteins.