FIG. 1.
MVA efficiently infects DC and induces TCD8+ specific for recombinant and viral antigens. (A) FACS analysis of MVA-GFP-infected mDC. Surface expression of MHC class I (HLA-A2), MHC class II (I-Ab), costimulatory molecules B7.1 (CD80) and B7.2 (CD86), and ICAM-1 (CD54) was analyzed for infected and noninfected mDC 6 h postinfection. (B) IFN-γ production of Tyr369-specific TCD8+ after stimulation with MVA-Tyr-infected mDC. (C) A*0201 mice were vaccinated i.p. with MVA-Tyr. Eight days later, splenocytes were incubated with the A*0201-restricted tyrosinase peptide Tyr369, the MVA-specific peptides A6L6, I1L211, and H3L184, or a control peptide and were permeabilized, stained with anti-CD8, anti-CD62L, and anti-IFN-γ antibodies, and then analyzed by flow cytometry. Depicted blots were gated on live TCD8+. Results are representative of more than three independent experiments. MVA-wt, wild-type MVA.