TABLE 1.
Detection of PSTVd infection in inoculated weed species from potato fields
| Plant speciesa | Family | PSTVd inoculum and detectionb
|
|||
|---|---|---|---|---|---|
| RNA
|
StyI cDNA fragments
|
||||
| Dot blot | RT-PCR | Dot blot | RT-PCR | ||
| Weed plant species | |||||
| Amaranthus retroflexus | Amaranthaceae | 8/0 | − | 8/3 | + |
| Atriplex sagittata | Amaranthaceae | 6/0 | − | 7/0 | − |
| Chenopodium album | Amaranthaceae | 7/0 | − | 7/0 | − |
| Anthemis arvensis | Asteraceae | 8/8 | + | 8/8 | + |
| Chamomilla recutita | Asteraceae | 8/7 | + | 8/8 | + |
| Sonchus arvensis | Asteraceae | 8/0 | − | 8/0 | − |
| Galinsoga parviflora | Asteraceae | 8/0 | − | 8/0 | − |
| Capsella bursa-pastoris | Brassicaceae | 6/0 | NP | 6/0 | − |
| Stellaria media | Caryophyllaceae | 8/0 | − | 8/0 | − |
| Erodium cicutarium | Geraniaceae | 7/0 | − | 8/0 | − |
| Plantago major | Plantaginaceae | 7/0 | NP | 7/0 | − |
| Veronica agrestis | Scrophulariaceae | 7/0 | − | 7/0 | +c |
| Indicator plant species | |||||
| Gynura aurantiaca | Asteraceae | 4/4 | + | 4/4 | + |
| Lycopersicon esculentum | Solanaceae | 8/8 | + | 6/6 | + |
| Nicotiana benthamiana | Solanaceae | 8/8 | + | 8/8 | + |
a
See Materials and Methods for full scientific names.
b
For RT-PCR analyses, mixed RNA samples were analyzed for viroid at 25 days p.i.: +, positive, and −, negative. For the dot blot analyses, the number of plants inoculated/number of plants infected is shown for 25 days p.i. NP, not performed.
c
Only a weak signal was detected.