TABLE 1.
Detected epitopesa
| Patient | Epitope sequence | Variant sequence | H77 position | HLA restrictionb |
|---|---|---|---|---|
| BR111 | NEGCGWAGW | 88-96 | B44 | |
| LLALLSCLTV | 178-187 | A2 | ||
| CINGVCWTV | 1073-1081 | A2 | ||
| KLVALGVNAV | 1406-1415 | A2 | ||
| ILAGYGAGV | 1851-1859 | A2 | ||
| VLSDFKTWL | 1987-1995 | A2 | ||
| RQVGDFHYV | 2094-2102 | A2 | ||
| ALYDVVSKL | 2594-2602 | A2 | ||
| BR554 | YLLPRRGPRL | 35-44 | A2 | |
| YRLWHYPCTI | 613-622 | Cw7 | ||
| LYGMWPLLL | 790-798 | Cw7 | ||
| CINGVCWTV | 1073-1081 | A2 | ||
| HAVGIFRAA | HVVGIFRAA | 1175-1183 | A2 | |
| LFFNILGGWV | 1807-1816 | A2 | ||
| VLSDFKTWL | 1987-1995 | A2 | ||
| SLLRHHNLVYSTTSRSA | SLLRNHNLVYSTTSRSA* | 2449-2465 | ||
| THFFSVLIARDQ | THLFSVLMARDQ* | 2847-2858 | ||
| 03-32 | NEGCGWAGW | 88-96 | (B44) | |
| LLALLSCLTV | 178-187 | (A2) | ||
| NASRCWVAM | 234-242 | B35 | ||
| RLADFAQGW | 461-469 | (B53) | ||
| NTRPPLGNWF | 541-550 | (B57) | ||
| YRLWHYPCTI | 613-622 | Cw7 | ||
| FYGMWPLLL | 790-798 | Cw7 | ||
| LSPYYKRYISW | 831-841 | (A25) | ||
| HPTLVFDITKL | 881-889 | |||
| AVFGPLWIL | 894-902 | |||
| CINGVCWTV | 1073-1081 | (A2) | ||
| VTRHADVIPV | 1133-1142 | (A2) | ||
| HAVGIFRAA | 1175-1183 | (A2/A68) | ||
| ATLGFGAYMSKA | 1260-1271 | A3 | ||
| GVDPNVRTGVRT | 1273-1284 | |||
| YDIIICDECHSTDATSIL | 1310-1327 | (B38) | ||
| HPNIEEVAL | HSNIEEVAL* | 1359-1367 | B35 | |
| ATDALMTGF | 1436-1444 | A1 | ||
| CVTQTVDFSLDPTFTIETTTL | 1457-1477 | |||
| TLTHPITK | 1636-1643 | B8 | ||
| LPYIEQGMML | 1715-1724 | B35 | ||
| VITPVVQTNW | 1745-1754 | |||
| LTTSQTLLF | 1801-1809 | (B57) | ||
| HRFAPPCKPLLR | 2131-2142 | (B27) | ||
| EPEPDVAVL | EPEPDVAW* | 2163-2171 | B35 | |
| LGVPPLRAWR | 2912-2921 | (B57) |
a
Epitope sequences and their positions according to the H77 reference sequence are indicated. Two peptide epitopes specifically synthesized according to the wild-type viral sequence are printed in italics in column 2. Variant peptides that resulted in reduced antigen recognition by ELISPOT titration assay are marked with an asterisk. The specific difference is indicated in boldface. No epitopes were detected for patient BR1427.
b
Known HLA restrictions for each epitope are given. Described HLA restrictions not matching our patients’ HLA types are given in parentheses.