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. 2007 Aug 22;81(21):11972–11981. doi: 10.1128/JVI.00617-07

FIG. 2.

FIG. 2.

Protein-protein blot analysis of AL2 self-interaction. (A) Immunoblot of total protein extracts from E. coli cells expressing either His-AL2 or His-CAT, probed with anti-His tag antibody, is shown. For experiments depicted in the remaining panels, three identical blots of partially purified GST and GST-AL2 were used: a blot probed with anti-GST antibody (B); a blot incubated with His-AL2-containing extract (primary probe) followed by being immunoblotted with anti-His tag antibody (secondary probe) (C); and a blot incubated with His-CAT-containing extract (primary probe, negative control) followed by being immunoblotted with anti-His tag antibody (secondary probe) (D). The positions of molecular mass standards are shown to the left of panel A. The positions of GST (∼26 kDa) and GST-AL2 (∼41 kDa) are indicated to the left of panels B and C. His-AL2 (∼16 kDa) consistently migrates at a slightly lower-than-expected rate in polyacrylamide gels. Additional faint bands observed in the GST-AL2 sample (B) are breakdown products.