FIG. 6.
CCHC mutant AL2 proteins localize to the nuclei of insect cells. Fractionated protein extracts from Sf9 cells infected with recombinant baculoviruses expressing either native TGMV AL2, CCHC mutant AL2, AL21-100, or CAT (a negative control protein) are shown. Nuclear fractions were prepared 48 h postinoculation, and equivalent amounts (representing 5 × 105 cells) were electrophoresed on 4 to 20% polyacrylamide-SDS gels and subjected to Western blot analysis using an AL2-specific antibody. The positions of nonphosphorylated and phosphorylated (asterisk) AL2 are indicated to the right. WT, wild type.