FIG. 7.

Local silencing suppression by AL2 and AL2 C33A. (A) Leaves of N. benthamiana line 16c, expressing a 35S-GFP transgene, were coinfiltrated with Agrobacterium cultures delivering Ti plasmids expressing GFP (silencing trigger) and AL2, AL2 C33A, GUS (negative control), or p19 (positive control). Representative leaves were photographed under UV light 6 days postinfiltration. Under these conditions, high-level GFP expression in the infiltration zone (relative to the level of GFP expression from the transgene) appears yellow-green. (B) Leaves of wild-type N. benthamiana plants were coinfiltrated with constructs expressing GFP, double-stranded GFP RNA (dsGFP) (silencing trigger), and AL2, AL2 C33A, or GUS (negative control). Total RNA was isolated from infiltration zones and was subjected to RNA gel blot hybridization using a ³²P-labeled GFP-specific probe. 18S rRNAs are shown as loading controls.