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Effect of helix disruption on the calcium-switch-mediated VLS formation by GFP-NSP5-C68. (A) Wild-type GFP-NSP5-C68 and mutants A182P, M185P, M192P, and L194P were subjected to a calcium switch as described in the legend to Fig. 6. (B) GFP-NSP5-C68 A191 was mutated to either Met or Pro, and the ability of mutants to respond to a calcium switch was visualized by fluorescence microscopy.
