FIG. 8.

Identification of functional DxDxD motifs in the NSP5 C terminus. (A) The C terminus of NSP5 contains two tandem DxDxD pseudo-EF-hand calcium motifs (in boldface) upstream of the predicted α-helix. The predicted NSP5 C-terminal α-helix is underlined. Alanine mutations introduced to disrupt DxDxD motifs individually are shown (DXM1 and DXM2), with mutations in boldface. (B) Expression of DXM1 and DXM2 mutant GFP-NSP5-C68 constructs following calcium starvation (upper row) or following exposure to high-calcium medium (lower row).