FIG. 1.

Time course of cytoplasmic remodeling. HLF cells were mock infected or infected for 144 h at an MOI of 0.01. The indicated organelle markers (ER, Bip/GRP78; Golgi, GM130; TGN, p230; and early endosomes, EEA1) were detected by confocal microscopy using MAb (red stain), and infected cells were detected with a rabbit polyclonal antibody to US18 (green). DNA was stained with DAPI. For each organelle and time point, the left panel is a merge of all three colors, and the right panel is a merge of just the green and blue channels.