FIG. 7.

Surface expression of G protein. (A) pTriEX1-GHis (a and c)- or pTriEX1 (b and d)-transfected LLC-MK2 cells were fixed with 4% paraformaldehyde and treated with 0.1% Triton X-100 for internal (a and b) or not treated for surface (c and d) immunofluorescence staining assay using anti-His and FITC-conjugated anti-mouse antibodies. (B) pTriEX1-GHis (lanes 1 to 4)- or empty vector (lane 5)-transfected cells were pulse-labeled for 10 min and chased for various times as indicated at the top. Cells were subsequently reacted with anti-His antibody, followed by extensive washing and cell lysis. The antibody-antigen complex was precipitated by using protein A-Sepharose. Eluted proteins were analyzed by electrophoresis, followed by autoradiography. Molecular size standards are indicated on the left.