FIG. 3.
SP does not inhibit the capture of HIV-1 mediated by DC-SIGN negative target cells. Activated PBMC (10 U of IL-2/ml plus 10 μg of PHA/ml for 48 h), monocytes, and the cell line SupT-1 were incubated for 30 min at 37°C without (controls) or with a SP dilution of 1:102 and then were exposed to HIV-1IIIB (CXCR4) (5 ng of p24) for 90 min at 37°C. The cells were then washed thoroughly, lysed, and evaluated for p24 antigen by ELISA. The data represent the arithmetic means ± the SEM of three experiments carried out in triplicate.