TABLE 3.
Titration and diameters of virus particles within single plaques isolated from either H262A mutant virus or MHV-A59 1000 in L2 cellsa
| Virus | Mean titer ± SEb (%) | Plaque diam (mm)c |
|---|---|---|
| MHV-A59 1000 | 5.64 × 105 ± 0.80 × 105 (100) | 2.22 ± 0.06 |
| vH262A | 6.76 × 104 ± 1.77 × 104 (12) | 2.26 ± 0.06 |
a
WT virus or vH262A was isolated from 12 individual plaques formed on L2 cells by using Pasteur pipettes, resuspended in 1 ml of DMEM, sonicated three times for 20 s at 35% power in a cup sonicator, and titered.
b
From 12 plaques.
c
Calculated as an average for at least 25 plaques. α is 0.05, and Tukey's W procedure was performed.