FIG. 4.

Internalization of gB in KgBLL871AA-infected cells. Seven hours after infection of 143B cultures by a mutant virus in which the gB LL motif was replaced by AA, cells were processed as described in the legend of Fig. 1. (A, E, I, M, and Q) Incubation for 0 min at 37°C; (B, F, J, N, and R) 15-min incubation at 37°C; (C, G, K, O, and S) 30-min incubation at 37°C; (D, H, L, P, and T) 60-min incubation at 37°C. Panels A to D show phase-contrast images. Anti-VP5, anti-TGN 46, and anti-gB staining is shown in panels E to H, I to L, and M to P, respectively. (Q to T) Merged images.