Virus growth. 143B cells were infected with KOS, KgBY889A, or KgBLL871AA, and the infectivity of the progeny viruses was analyzed at different times postinfection by titration on Vero cells. (A) Cells were infected at an MOI of 5, and extra- and intracellular infectivities were assayed at 6, 9, 12, 16, 20, and 24 h postinfection. (B) Multiple-step growth analysis. Cells were infected at an MOI of 0.001, and progeny viruses were harvested 24, 48, and 72 h postinfection. The data shown correspond to averages of two or three independent experiments. Vertical lines indicate the standard deviations.