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Incorporation of gB in virions. Extracellular virus recovered from infected cell supernatants at 8 h postinfection were subjected to polyacrylamide gel electrophoresis under denaturing conditions and transferred onto nitrocellulose membrane. The membrane was treated with anti-gB and anti-ICP5 antibodies and then with HRP-coupled secondary antibodies visualized by enhanced chemiluminescence. Quantification analysis was performed with Fujifilm Multi-Gauge software.
