FIG. 1.

Cardiac myocytes express higher basal IFN-β mRNA and nuclear activated ISGF3 components and have higher ISG561 mRNA expression than cardiac fibroblasts. RNA and nuclear protein extracts were harvested from primary cardiac myocyte (Myo) and cardiac fibroblast (Fib) cultures at 2 days postplating. Cultures were generated from wild-type mice, unless indicated otherwise (IFN-α/β-RKO mice). RNA was analyzed by real-time RT-PCR, and copy number was normalized to GAPDH. (A) Basal IFN-β mRNA. (B) Basal ISG561 mRNA. Graphs are representatives of three independent experiments from three independently derived myocyte and fibroblast cultures ± standard deviations. The asterisk indicates a difference at P < 0.05. (C) Western blot analysis of basal nuclear activated ISGF3 components.