FIG. 7.

Cardiac fibroblasts are more responsive than cardiac myocytes to IFN for induction of STAT phosphorylation. Two days postplating, primary cardiac cell cultures were treated with increasing doses of IFN-α/β and at 30 min and 2 h post-IFN treatment total protein extracts were harvested. Proteins were separated by SDS-PAGE, transferred to a nylon membrane, and probed using the indicated antibodies. Results are representative of multiple experiments.