FIG. 9.

IFN-β-dependent inhibition of T3D replication is greater in cardiac fibroblasts than in cardiac myocytes. One day postplating, primary cardiac cell cultures were pretreated with IFN-β (10, 100, or 1,000 U/ml). Twenty-four hours post-IFN-β treatment, cultures were infected with T3D at 5 PFU per cell. Control-treated cultures received 1,000 neutralizing units/ml of rabbit polyclonal anti-mouse IFN-β antibody. At 12 h and 24 h postinfection, cell cultures were frozen and titers were determined by plaque assay. (A) Viral titers, expressed as PFU per well ± standard deviations. (B) Data from panel A are expressed as percentages of PFU in IFN-β-treated cultures relative to control (anti-IFN-β)-treated cultures ± standard deviations. At every time point, differences between myocytes and fibroblasts were statistically significant (P < 0.05).