TABLE 1.
Effect of MG-132 on virus production
| Treatmenta | Virus titerb (fold decreasec)
|
|
|---|---|---|
| VSV | SFV | |
| None | 1.6 × 109 | 5.2 × 109 |
| DMSO | 1.0 × 109 | 3.4 × 109 |
| MG-132 | 3.4 × 106 (290) | 2.8 × 109 (1.2) |
a
BHK-21 cells were infected with VSV or SFV at 10 PFU/cell for 1 h, washed, cultured for an additional 2 h, and then incubated in the presence of the carrier DMSO or 50 μM MG-132 as indicated.
b
In PFU per milliliter. Media from infected cells were collected 5 h after addition of MG-132. Virus titers were determined by a plaque assay. The average titer from duplicate plaque assays from one experiment is shown.
c
Compared to the value for the DMSO control.