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HA-VP24 interacts with endogenous karyopherin α1. 293T cells were transfected with either FLAG-VP24 or FLAG-VP35, and 1 day posttransfection immunoprecipitation was performed with the anti-FLAG monoclonal antibody M2 bound to agarose beads. The immunoprecipitated material (FLAG-IP) and whole-cell extract (WCE) were then subjected to Western blot analysis with a monoclonal antibody against karyopherin α1 (Kα1) and an anti-FLAG monoclonal antibody.
