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. 2007 Oct 10;81(24):13469–13477. doi: 10.1128/JVI.01097-07

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Copyright © 2007, American Society for Microbiology

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FIG. 5. — Reston and MA VP24 proteins interact with the NPI-1 subfamily of karyopherin α and inhibit the interaction of PY-STAT1 with karyopherin. (A) 293T cells were transfected with FLAG-Kα1, FLAG-Kα2, FLAG-Kα5, or FLAG-Kα6 alone or along with either HA-ZEBOV VP24 (HA-VP24), HA-REBOV VP24 (HA-R-VP24), or (B) HA MA ZEBOV (HA-MA-VP24). One day posttransfection the cells were treated 1,000 U/ml of IFN-β for 30 min and then subjected to immunoprecipitation with the anti-FLAG monoclonal antibody. The immunoprecipitated (IP) material and whole-cell extract (WCE) were then subjected to Western blot analysis with the polyclonal rabbit antiserum recognizing PY-STAT1, FLAG, and an anti-HA monoclonal antibody. The asterisk indicates the light chain of the anti-FLAG monoclonal M2 antibody used for the immunoprecipitation.