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. 2007 Oct 10;81(24):13469–13477. doi: 10.1128/JVI.01097-07

FIG. 6.

FIG. 6.

EBOV VP24 proteins inhibit reporter gene expression in human and murine cells. 293T (A), HuH7 (B), NIH-3T3 (C), or MEF (D) cells were transfected with the ISG54-CAT reporter plasmid, a constitutively expressed Renilla luciferase reporter plasmid, and either empty vector (vector) or the indicated plasmids expressing various concentrations (a, 500, 100, 20, and 4 ng; b, 500, 100, 20, and 4 ng; c, 500, 100, and 20 ng; and d, 500, 100, and 20 ng) of ZEBOV VP24 (Z-VP24), MA ZEBOV VP24 (MA-VP24), or REBOV VP24 (R-VP24). At 1 day posttransfection the cells were either mock treated or treated with 1,000 U/ml of universal type I IFN or human IFN-β (for 293T and HuH7 cells) or murine IFN-β (for NIH-3T3 cells and MEFs). At 16 h posttreatment the cells were harvested and assayed for reporter activities.