TABLE 1.
Primers used for construction of the HCV core-expressing plasmidsa
| Plasmid name | Direction | Primer sequence |
|---|---|---|
| pCXbsr/core(HCV-O) | Forward | 5′-GGAATTCCCACCATGAGCACGAATCCTAAACCTC-3 |
| Reverse | 5′-ATAAGAATGCGGCCGCCTATCAAGCGGAAGCTGGGATGGT-3′ | |
| pcDNA3/core(HCV-O) | Forward | 5′-CGGGATCCAAGATGAGCACGAATCCTAAACCTCAAAGA-3′ |
| pcDNA3/FLAG-core(HCV-O) | Reverse | 5′-CCGCTCGAGTCAAGCGGAAGCTGGGATGGTCAAACA-3′ |
| pcDNA3/Δcore(HCV-O) | Forward | 5′-CGGGATCCAAGATGGGCCCCAGGTTGGGTGTGCGC-3′ |
| pcDNA3/FLAG-Δcore(HCV-O) | Reverse | 5′-CCGCTCGAGTCAAGCGGAAGCTGGGATGGTCAAACA-3′ |
| pcDNA3/core(JFH1) | Forward | 5′-CGGGATCCAAGATGAGCACAAATCCTAAACCTCAAAGA-3′ |
| pcDNA3/FLAG-core(JFH1) | Reverse | 5′-CCGCTCGAGTCAAGCAGAGACCGGAACGGTGATGCA-3′ |
| pcDNA3/Δcore(JFH1) | Forward | 5′-CGGGATCCAAGATGGGCCCCAGGTTGGGTGTGCGC-3′ |
| pcDNA3/FLAG-Δcore(JFH1) | Reverse | 5′-CCGCTCGAGTCAAGCAGAGACCGGAACGGTGATGCA-3′ |
To construct pCXbsr/core(HCV-O), a DNA fragment encoding the core was amplified by PCR from pON/C-5B (13) with the indicated primers. The PCR product was digested with EcoRI-NotI and subcloned into the same site of pCX4bsr (1). To construct pcDNA3/core(HCV-O), pcDNA3/FLAG-core(HCV-O), pcDNA3/Δcore(HCV-O), and pcDNA3/FLAG-Δcore(HCV-O), DNA fragments encoding the core were amplified by PCR from pON/C-5B (13) with the indicated primer sets. To construct pcDNA3/core(JFH1), pcDNA3/FLAG-core(JFH1), pcDNA3/Δcore(JFH1), and pcDNA3/FLAG-Δcore(JFH1), DNA fragments encoding the core were amplified by PCR from pJFH1 (23) with the indicated primer sets. The PCR products were digested with BamHI and XhoI and then subcloned into the same site of pcDNA3 (Invitrogen) or pcDNA3-FLAG (2).