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Replication of Δm01-17+m144-158-MCMV and WT-MCMV in vitro. NIH 3T3 fibroblasts (a), Ana-1 macrophages (b), SVEC4-10 endothelial cells (c), and C127 mammary epithelial cells (d) were infected in triplicate with Δm01-17+m144-158-MCMV or WT-MCMV at an MOI of 0.1. Virus in the supernatant was harvested every 24 h, from day 0 (input virus) to day 4 or 5 p.i., and infectious virus titers were determined by plaque assay with MEF. Values shown are means ± standard deviations.
