FIG. 1.

Effect of PI-88 or heparin on infectivity of PI-88 escape variants of HSV-2. Approximately 100 to 200 PFU of a specific AC variant (A and B) or an A or C variant (C and D) were mixed with PI-88 (0.16 to 100 μg/ml) or heparin (0.16 to 100 μg/ml) and incubated for 15 min at room temperature prior to being added to and incubated with GMK AH1 cells for 2 h at 37°C. The cells were then washed and overlaid with methylcellulose solution without an inhibitor. The results are expressed as percentages of the number of viral plaques of the PI-88/heparin-treated virus relative to that of the mock-treated controls. The values shown are the means of duplicate determinations from two separate experiments.