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Infection of GMK AH1 cells with the gG-deficient mutant of HSV-2. The cells were infected with the gG-deficient recombinant virus 333+AC6gG (A and C) or the gG-proficient 333(gG+) strain (B and D) at a multiplicity of infection of 0.001. The cells were washed and maintained in Eagle's medium without methylcellulose or human gamma globulin. The cells were immunostained with the monoclonal anti-HSV gB antibody B11D8 at 24 h (A and B) or 48 h (C and D) after infection.
