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. 2007 Oct 10;81(24):13578–13586. doi: 10.1128/JVI.01663-07

FIG. 1.

FIG. 1.

XBP-1 is unspliced in PEL, but splicing is induced by ER stress. (A) RT-PCR amplification across the XBP-1 intron produces a 249-base-pair amplicon from XBP-1u mRNA and a 223-base-pair amplicon from XBP-1s mRNA. PstI digests only the XBP-1u amplicon. RT-PCR amplification from the Burkitt's lymphoma cell line Raji, the PEL cell lines BC-3, BCBL-1, JSC-1, and HBL-6, and the multiple myeloma cell line SK-MM-2 mRNA shows that XBP-1s is produced in SK-MM-2 cells. In these cells, a slower-migrating, non-PstI-digestible PCR hybrid between XBP-1s and XBP-1u products is visible, similar to hybrids described previously (37). (B) RT-PCR amplification from BC-3 or JSC-1 cells mRNA cultured normally or treated for 1 h with 2 mM DTT. XBP-1s and XBP-1u controls were amplified from pXBPsIG and pXBPuIG, respectively. Composite images of the same gel are shown.