FIG. 6.

Maturation of the oligosaccharides N-linked to the 8ab⁺ protein. vTF7-3 infected OST7-1 cells were transfected with constructs encoding 8ab⁺ or 8ab⁺-EGFP. The cells were labeled with ³⁵S-labeled amino acids from 5 to 6 h p.i., lysed directly (pulse), or chased for 2 h before lysis (chase), and processed for immunoprecipitation with rabbit antiserum against 8ab⁺ or the EGFP tag. Precipitated immunocomplexes were treated with PNGase F (F) or endo H (H) or were mock treated (−) and then subjected to SDS-15% PAGE analysis. The positions and masses (in kDa) of the molecular mass protein markers are indicated. Only the relevant portions of the gels are shown.