TABLE 1.
Primer no. (description)a | Sequence (5′ to 3′)b | Polarity | Purpose |
---|---|---|---|
2220 (8a rev SOE) | ccattcaggttggtaaccagtaggACAAGGATCTTCAAGCACAT | − | 8ab+ |
2221 (8b for SOE) | ctggttaccaacctgaatggaatatAAGGTACAACACTAGGGGTAA | + | 8ab+ |
2267 (8b for) | gaattcaccATGTGCTTGAAGATCCT | + | 8b-EGFP |
2985 (8a for) | gaattcaccATGAAACTTCTCATTGTTTT | + | 8a-EGFP, 8abΔ-EGFP, 8ab+, 8ab+-EGFP |
2268 (8b rev) | ggatccTTAATTTGTTCGTTTATTT | − | 8ab+ |
3191 (8a rev stop) | ggatccGTGTTGTACCTTACAAGGA | − | 8a-EGFP |
2986 (8b rev stop) | ggatccATTTGTTCGTTTATTTAAAAC | − | 8b-EGFP, 8abΔ-EGFP, 8ab+-EGFP |
3207 (leader for) | ctcgagaccATATTAGGTTTTTACCTACC | + | L-8ab+-EGFP, L-8abΔ-EGFP |
3208 (leader rev) | gaagtttcatGTTCGTTTAGAGAACAGATCT | − | L-8ab+-EGFP, L-8abΔ-EGFP |
3209 (TRS 8a for) | TCTAAACGAACATGAAACTTCT | + | L-8ab+-EGFP, L-8abΔ-EGFP |
3043 (8a for −ss) | gaattcaccATGATATGCACTGTAGTACAGCG | + | 8ab+,−ss, 8ab+,−ss-EGFP |
3212 (EGFPglyc for) | GCGACGTAAACGGCacCAAGTTCAGCGTG | + | N-glycosylation site in EGFP |
3213 (EGFPglyc rev) | CACGCTGAACTTGgtGCCGTTTACGTCGC | − | N-glycosylation site in EGFP |
for, forward primer; rev, reverse primer.
Coding sequences are shown in uppercase. Lowercase letters indicate nucleotides added for cloning purposes, with the restriction enzyme recognition sites underlined.