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. 2007 Oct 10;81(24):13876–13888. doi: 10.1128/JVI.01631-07

TABLE 1.

Sequence, polarity, and purpose of primers used in this study

Primer no. (description)a Sequence (5′ to 3′)b Polarity Purpose
2220 (8a rev SOE) ccattcaggttggtaaccagtaggACAAGGATCTTCAAGCACAT 8ab+
2221 (8b for SOE) ctggttaccaacctgaatggaatatAAGGTACAACACTAGGGGTAA + 8ab+
2267 (8b for) gaattcaccATGTGCTTGAAGATCCT + 8b-EGFP
2985 (8a for) gaattcaccATGAAACTTCTCATTGTTTT + 8a-EGFP, 8abΔ-EGFP, 8ab+, 8ab+-EGFP
2268 (8b rev) ggatccTTAATTTGTTCGTTTATTT 8ab+
3191 (8a rev stop) ggatccGTGTTGTACCTTACAAGGA 8a-EGFP
2986 (8b rev stop) ggatccATTTGTTCGTTTATTTAAAAC 8b-EGFP, 8abΔ-EGFP, 8ab+-EGFP
3207 (leader for) ctcgagaccATATTAGGTTTTTACCTACC + L-8ab+-EGFP, L-8abΔ-EGFP
3208 (leader rev) gaagtttcatGTTCGTTTAGAGAACAGATCT L-8ab+-EGFP, L-8abΔ-EGFP
3209 (TRS 8a for) TCTAAACGAACATGAAACTTCT + L-8ab+-EGFP, L-8abΔ-EGFP
3043 (8a for −ss) gaattcaccATGATATGCACTGTAGTACAGCG + 8ab+,−ss, 8ab+,−ss-EGFP
3212 (EGFPglyc for) GCGACGTAAACGGCacCAAGTTCAGCGTG + N-glycosylation site in EGFP
3213 (EGFPglyc rev) CACGCTGAACTTGgtGCCGTTTACGTCGC N-glycosylation site in EGFP
a

for, forward primer; rev, reverse primer.

b

Coding sequences are shown in uppercase. Lowercase letters indicate nucleotides added for cloning purposes, with the restriction enzyme recognition sites underlined.