FIG. 9.

Reduced Rb and MCM5 complexes with E2F1 in the infected cells. (A) E2F1 was immunoprecipitated with anti-E2F1 antibody, starting with 500 μg lysate. Proteins, which were coprecipitated, were tested using antibodies to Rb, MCM5, and γ-tubulin. (B) Graphic representation of the results relative to the highest level of protein expression in the mock-infected cells. The quantitative analyses were based on results from two independent experiments. The differences of Rb and MCM5 between mock-infected and virus-infected cells were statistically significant, as determined by t tests.