FIG. 2.

Protease treatment enhances infectivity of Ebola virus GP pseudovirions. HIV-Ebola virus GP virions were treated with 10 μg/ml CatL (A) or 0.5 mg/ml thermolysin (B) at 37°C for the indicated times. Reactions were terminated as described for Fig. 1. Equal volumes of mock- or protease-treated virion preparations were used to infect VeroE6 cells. Luciferase activity was measured 48 h postinfection. Results are reported in relative light units (RLU) and are the means and standard deviations for samples run in triplicate. Similar results were obtained with 293T cells as infectious targets (data not shown).