Skip to main content
. 2007 Oct 10;81(24):13378–13384. doi: 10.1128/JVI.01170-07

FIG. 4.

FIG. 4.

Cell binding and infectivity of mucin deletion Ebola virus GP pseudovirions. HIV p24 normalized HIV-Ebola virus GP-Δmuc or HIV-Ebola virus wild-type GP pseudovirions were mock treated or treated with CatL for 60 min, and the reactions were quenched at the indicated times. (A) Ebola virus GP-Δmuc samples were analyzed by immunoblotting using polyclonal GP1 antisera. (B) Samples were added to VeroE6 cells and incubated at 37°C for 48 h. Infection was determined by measuring luciferase activity in cell lysates. Results are reported as luciferase activity relative to the no-enzyme control. Mock-treated Ebola virus wild-type GP, 40 relative light units; mock-treated Ebola virus GP-Δmuc, 190 relative light units. (C) Proteolysed virions were bound to VeroE6 cells, and the amount of p24 recovered from cell lysates relative to the input was determined by p24 ELISA.