FIG. 5.

Cathepsin proteolysis of Ebola virus GP enhances binding of virions to Jurkat cells, but resistance to infection is maintained. HIV-Ebola virus GP virions were mock treated or treated with CatL for 30 min and bound to Jurkat or VeroE6 cells at 4°C. A portion of the samples was immediately washed, lysed, and subjected to p24 ELISA (A). The remaining samples were incubated at 37°C for 48 h and analyzed for infection (B). Infection is reported as the percent GFP-positive cells, as determined by flow-cytometric analysis. Results are the means and standard deviations for samples run in triplicate.