FIG. 5.

MEFs use the MAVS pathway to induce type I IFN production in response to HSV infection. (A and B) MEFs from C57BL/6 or MAVS^−/− mice were seeded and infected with 3 × 10⁶ PFU of HSV-1 or HSV-2/ml. After 24 and 4 h, supernatants and total RNA were harvested for measurement of the IFN-α/β bioactivity (A) and mRNA (B), respectively. (C) MEFs from C57BL/6 or MAVS^−/− mice were seeded and transfected with calf thymus-derived DNA using Lipofectamine 2000. Twenty-four hours later the supernatants were harvested for measurement of the IFN-α/β bioactivity. (D) MEFs from C57BL/6 mice were seeded and infected with 3 × 10⁶ PFU/ml of the 17+ or KOS strains of HSV-1 and the indicated mutants. After 24 h, the supernatants were harvested, and the IFN-α/β levels were measured. For all data in this figure, similar results were obtained in three independent experiments. Error bars indicate the SEM.