FIG. 6.

The RRM3 domain is important for splicing in vivo. (A to C) Diverse response (partial or complete splicing inhibition or efficient splicing) of the RRM3 ts mutant in the splicing of the endogenous transcripts as described for Fig. 1 to 3. (D) Increasing the Py tract length reduces RRM3 dependence in vivo. Splicing patterns of cdc2.2 and the cdc2.2 Py tract variant introns (expressed from plasmids) in various strains incubated for 0, 1, and 2 h at 37°C are shown. The Py tract region of the cdc2.2 wt intron was replaced by a uridine tract (Y-long). Dashes indicate identical nucleotides.