Skip to main content
. 2007 Aug 20;27(20):7220–7235. doi: 10.1128/MCB.00274-07

FIG. 6.

FIG. 6.

The PLU-1/JARID1B domain specifically binds the GCACA consensus sequence. (A) Sequence details of the oligonucleotide variants used in the EMSAs. The consensus and variant sequences are shown in bold, while the mutated bases in the variant sequences are underlined. (B and C) EMSAs were performed using purified bacterially expressed GST/PLU-1-ARID domain, labeled probe corresponding to PLU-1/JARID1B consensus sequence (2× GCACA), and indicated cold competitor oligonucleotides at 5-, 10-, and 20-fold molar excesses. A fivefold molar excess of cold consensus oligonucleotide is able to compete away most of the consensus oligonucleotide, while the consensus site variants are poor competitors. The AATTAAA sequence is a better competitor than the variant. Arrows indicate the position of DNA/GST-PLU-1/ARID complex.